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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
03/02/2020 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MONTENEGRO, M.C.; PERAZA, P.; BALEMIAN, N.; CARBALLO, C.; BARLOCCO, N.; BARRIOS, P.G.; MERNIES, B.; SAADOUN, A.; CASTRO, G.; GUIMARÃES, S.F.; LLAMBÍ, S. |
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Afiliación : |
M. C. MONTENEGRO, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; PABLO PERAZA DOS SANTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. BALEMIAN, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; C. CARBALLO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; N. BARLOCCO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; P. G. BARRIOS, Departamento de Biometría, Estadística y Cómputo, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; B. MERNIES, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; A. SAADOUN, Sección Fisiología y Nutrición, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; G. CASTRO, Departamento Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; S.F. GUIMARÃES, Departamento de Zootecnia, Universidade Federal de Viçosa, Minas Gerais, Brasil; S. LLAMBÍ, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Gene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Genetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307 |
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ISSN : |
1676-5680 |
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DOI : |
10.4238/gmr18307 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019.
Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com |
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Contenido : |
ABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits. MenosABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regu... Presentar Todo |
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Palabras claves : |
Candidate genes; Meat quality; Nutrigenomics; Skeletal muscle; Transcriptomic. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://www.geneticsmr.com/sites/default/files/articles/year2019/vol18-4/pdf/gmr18307_-_gene-expression-analysis-rna-sequencing-longissimus-dorsi-muscle-pigs-fed-diets-differing.pdf
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Marc : |
LEADER 03091naa a2200337 a 4500
001 1060743
005 2022-07-20
008 2019 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/gmr18307$2DOI
100 1 $aMONTENEGRO, M.C.
245 $aGene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents.$h[electronic resource]
260 $c2019
500 $aArticle history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019. Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com
520 $aABSTRACT. We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments. These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits.
653 $aCandidate genes
653 $aMeat quality
653 $aNutrigenomics
653 $aSkeletal muscle
653 $aTranscriptomic
700 1 $aPERAZA, P.
700 1 $aBALEMIAN, N.
700 1 $aCARBALLO, C.
700 1 $aBARLOCCO, N.
700 1 $aBARRIOS, P.G.
700 1 $aMERNIES, B.
700 1 $aSAADOUN, A.
700 1 $aCASTRO, G.
700 1 $aGUIMARÃES, S.F.
700 1 $aLLAMBÍ, S.
773 $tGenetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/09/2022 |
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Actualizado : |
27/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
HECKER, Y. P.; BURUCÚA, M. M.; FIORANI, F.; MALDONADO RIVERA, J. E.; CIRONE, K.M.; DORSCH, M.; CHEUQUEPÁN, F. A.; CAMPERO, L. M.; CANTÓN, G. J.; MARÍN, M. S.; ORTEGA-MORA, L. M.; MOORE, D. P. |
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Afiliación : |
YANINA P. HECKER, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, 7620, Argentina; MERCEDES M.BURUCÚA, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, 7620, Argentina; FRANCO FIORANI, Inst. Innov. Prod. Agropecuaria y Desarrollo Sostenible (IPADS), Inst. Nac. Tecnol. Agropecuaria (INTA)—Consejo Nac. Inv. Científicas y Técnicas (CONICET), Balcarce, Argentina; Fac. Ciencias Agrarias, Univ. Nac. Mar del Plata, Balcarce, Argentina; JAIME E. MALDONADO RIVERA, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Argentina; Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca, Ecuador; KARINA M. CIRONE, Inst. Innov. Prod. Agropecuaria y Desarrollo Sostenible (IPADS), Inst. Nac. Tecnol. Agropecuaria (INTA)?Consejo Nac. Inv. Científicas y Técnicas (CONICET), Balcarce, Argentina; Fac. Ciencias Agrarias, Univ. Nac. Mar del Plata, Balcarce, Argentina; MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Argentina; FELIPE A. CHEUQUEPÁN, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; LUCÍA M. CAMPERO, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; GERMÁN J. CANTÓN, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; MAIA S. MARÍN, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; LUIS M. ORTEGA-MORA, SALUVET, Departamento de Sanidad Animal, Facultad de Ciencias Veterinarias, Universidad Complutense de Madrid, Madrid, Spain; DADÍN P. MOORE, Inst. Innov. Prod. Agropecuaria y Desarrollo Sostenible (IPADS), Inst. Nac. Tecnol. Agropecuaria (INTA)?Consejo Nac. Inv. Científicas y Técnicas (CONICET), Balcarce, Argentina; Fac. Ciencias Agrarias, Univ. Nac. Mar del Plata, Balcarce, Argentina. |
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Título : |
Reactivation and foetal infection in pregnant heifers infected with Neospora caninum live tachyzoites at prepubertal age. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Vaccines, 2022, Volume 10, Issue 8, article 1175. OPEN ACCESS. doi: https://doi.org/10.3390/vaccines10081175 |
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ISSN : |
2076-393X |
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DOI : |
10.3390/vaccines10081175 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 5 July 2022; Revised 14 July 2022; Accepted 22 July 2022; Published 25 July 2022.
Corresponding author: Hecker, Y.P.; Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; email:hecker.yanina@inta.gob.ar --
Article: Gold Open Access, Green Open Access. -- Academic Editor: S. Louise Cosby. -- This article belongs to the Special Issue Vaccines in Farm Animals: https://www.mdpi.com/journal/vaccines/special_issues/Farm_Vaccines -- |
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Contenido : |
ABSTRACT.- Neospora caninum is recognised for causing cattle abortion, provoking severe economic losses in the livestock industry worldwide. The aim of the present study was to evaluate the reactivation and foetal infection in pregnant heifers inoculated with live N. caninum tachyzoites before puberty. A total of 15 30-month-old pregnant heifers were allocated into four groups: animals inoculated with live tachyzoites of NC-Argentina LP1 isolate before puberty and challenged with live tachyzoites of NC-1 strain at 210 days of gestation (DG) (Group A); animals mock inoculated before puberty and challenged with NC-1 strain at 210 DG (Group B), animals inoculated before puberty but not subsequently challenged (Group C); and noninfected and nonchallenged animals (Group D). The results of this study showed that 100% of animals infected before puberty (Groups A and C) suffered reactivation of the infection at the seventh month of gestation. In addition, in three and two calves from Groups A and C, respectively, congenital infection was confirmed. Interestingly, we provide evidence that the use of live N. caninum tachyzoites in young animals as a strategy to induce protection is neither safe nor effective. © 2022 by the authors. |
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Palabras claves : |
Bovine; Efficacy; Live parasite; Neospora caninum; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA SALUD ANIMAL; Prepubertal inoculation; Reactivation; Safety; Vertical transmission. |
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Asunto categoría : |
L50 Fisiología y bioquímica animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16773/1/vaccines-10-01175-v2.pdf
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Marc : |
LEADER 03144naa a2200409 a 4500
001 1063582
005 2022-09-27
008 2022 bl uuuu u00u1 u #d
022 $a2076-393X
024 7 $a10.3390/vaccines10081175$2DOI
100 1 $aHECKER, Y. P.
245 $aReactivation and foetal infection in pregnant heifers infected with Neospora caninum live tachyzoites at prepubertal age.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 5 July 2022; Revised 14 July 2022; Accepted 22 July 2022; Published 25 July 2022. Corresponding author: Hecker, Y.P.; Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; email:hecker.yanina@inta.gob.ar -- Article: Gold Open Access, Green Open Access. -- Academic Editor: S. Louise Cosby. -- This article belongs to the Special Issue Vaccines in Farm Animals: https://www.mdpi.com/journal/vaccines/special_issues/Farm_Vaccines --
520 $aABSTRACT.- Neospora caninum is recognised for causing cattle abortion, provoking severe economic losses in the livestock industry worldwide. The aim of the present study was to evaluate the reactivation and foetal infection in pregnant heifers inoculated with live N. caninum tachyzoites before puberty. A total of 15 30-month-old pregnant heifers were allocated into four groups: animals inoculated with live tachyzoites of NC-Argentina LP1 isolate before puberty and challenged with live tachyzoites of NC-1 strain at 210 days of gestation (DG) (Group A); animals mock inoculated before puberty and challenged with NC-1 strain at 210 DG (Group B), animals inoculated before puberty but not subsequently challenged (Group C); and noninfected and nonchallenged animals (Group D). The results of this study showed that 100% of animals infected before puberty (Groups A and C) suffered reactivation of the infection at the seventh month of gestation. In addition, in three and two calves from Groups A and C, respectively, congenital infection was confirmed. Interestingly, we provide evidence that the use of live N. caninum tachyzoites in young animals as a strategy to induce protection is neither safe nor effective. © 2022 by the authors.
653 $aBovine
653 $aEfficacy
653 $aLive parasite
653 $aNeospora caninum
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA SALUD ANIMAL
653 $aPrepubertal inoculation
653 $aReactivation
653 $aSafety
653 $aVertical transmission
700 1 $aBURUCÚA, M. M.
700 1 $aFIORANI, F.
700 1 $aMALDONADO RIVERA, J. E.
700 1 $aCIRONE, K.M.
700 1 $aDORSCH, M.
700 1 $aCHEUQUEPÁN, F. A.
700 1 $aCAMPERO, L. M.
700 1 $aCANTÓN, G. J.
700 1 $aMARÍN, M. S.
700 1 $aORTEGA-MORA, L. M.
700 1 $aMOORE, D. P.
773 $tVaccines, 2022, Volume 10, Issue 8, article 1175. OPEN ACCESS. doi: https://doi.org/10.3390/vaccines10081175
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